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Western blot analysis of extracts from MCF-7 cells lyastes.using SA2 Rabbit mAb [HKV3] at dilution of 1:1000 incubated at 4℃ over night
Protein names :STAG2,SA2,STAG2_HUMAN,Cohesin subunit SA-2
UniProtID :Q8N3U4
MASS(da) :141,326
MW(kDa) :141 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human SA2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells lyastes
Function : Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis..
Subcellular locationi :Nucleus. Chromosome. Chromosome, centromere.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.