Bad (S112) Rabbit mAb [34NH]Cat NO.: A25104

Protein names ：BAD,BBC6,BCL2L8,BAD_HUMAN,Bcl2-associated agonist of cell death

UniProtID ：Q92934

MASS(da) ：18,392

MW(kDa) ：18 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫荧光(ICC/IF),
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser112 of Human Bad .

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：C6 cells lysates CA-treated

Function ： Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways..

Tissue specificity ：Expressed in a wide variety of tissues.

Subcellular locationi ：Mitochondrion outer membrane. Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.