ASP beta-hydroxylase Rabbit mAb [C138]Cat NO.: A69950

Protein names ：ASPH,BAH,ASPH_HUMAN,Aspartyl/asparaginyl beta-hydroxylase

UniProtID ：Q12797

MASS(da) ：85,863

MW(kDa) ：120-130 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100,
• 免疫荧光(ICC/IF)1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of Human ASP beta-hydroxylase.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells lyastes

Function ： [Isoform 1]: Specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.., [Isoform 8]: Membrane-bound Ca(2+)-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells..

Tissue specificity ：Isoform 1 is detected in all tissues tested. Isoform 8 is mainly expressed in pancreas, heart, brain, kidney and liver. Isoform 8 is expressed in kidney (at protein level)..

Subcellular locationi ：[Isoform 1]: Endoplasmic reticulum membrane,Single-pass type II membrane protein.,[Isoform 4]: Sarcoplasmic reticulum membrane,Single-pass type II membrane protein.,[Isoform 8]: Endoplasmic reticulum membrane,Single-pass type II membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.