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Western blot analysis of extracts from Caco-2 cells lyastes.using ATP7b Rabbit mAb [RHAK] at dilution of 1:1000 incubated at 4℃ over night
Protein names :ATP7B,PWD,WC1,WND,ATP7B_HUMAN,Copper-transporting ATPase 2 [Cleaved into: WND/140 kDa]
UniProtID :P35670
MASS(da) :157,263
MW(kDa) :157 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ATP7b.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Caco-2 cells lyastes
Function : Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload..
Tissue specificity :Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
Subcellular locationi :Golgi apparatus, trans-Golgi network membrane,Multi-pass membrane protein. Late endosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.