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Western blot analysis of extracts from A549 cells lyastes.using Glypican 5 Rabbit mAb [1669] at dilution of 1:1000 incubated at 4℃ over night
Protein names :GPC5,GPC5_HUMAN,Glypican-5 [Cleaved into: Secreted glypican-5]
UniProtID :P78333
MASS(da) :63,707
MW(kDa) :63 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Glypican 5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells lyastes
Function : Cell surface proteoglycan that bears heparan sulfate..
Tissue specificity :In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver..
Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor,Extracellular side.,[Secreted glypican-5]: Secreted, extracellular space.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.