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Western blot analysis of extracts from U-87 MG cells lyastes.using PSMG1 Rabbit mAb [F2IU] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PSMG1,C21LRP,DSCR2,PAC1,PSMG1_HUMAN,Proteasome assembly chaperone 1
UniProtID :O95456
MASS(da) :32,854
MW(kDa) :33 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PSMG1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-87 MG cells lyastes
Function : Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization..
Tissue specificity :In the adult, detected in brain, colon, leukocytes, breast and testis. Widely expressed in the fetus. Also expressed in a variety of proliferating cell lines..
Subcellular locationi :Cytoplasm. Endoplasmic reticulum.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.