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PSMG1 Rabbit mAb [F2IU]Cat NO.: A85831

Western blot analysis of extracts from U-87 MG cells lyastes.using PSMG1 Rabbit mAb [F2IU] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :PSMG1,C21LRP,DSCR2,PAC1,PSMG1_HUMAN,Proteasome assembly chaperone 1

UniProtID :O95456

MASS(da) :32,854

MW(kDa) :33 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PSMG1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :U-87 MG cells lyastes

Function : Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization..

Tissue specificity :In the adult, detected in brain, colon, leukocytes, breast and testis. Widely expressed in the fetus. Also expressed in a variety of proliferating cell lines..

Subcellular locationi :Cytoplasm. Endoplasmic reticulum.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.