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Western blot(SDS PAGE) analysis of extracts from HeLa cells Arsenic-treated.Using HO-1 Rabbit mAb [0X39] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HMOX1,HO,HO1,HMOX1_HUMAN,Heme oxygenase 1 [Cleaved into: Heme oxygenase 1 soluble form]
UniProtID :P09601
MASS(da) :32,819
MW(kDa) :28 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human HO-1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells Arsenic-treated
Function : [Heme oxygenase 1]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:7703255, PubMed:11121422). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707).., [Heme oxygenase 1 soluble form]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron..
Tissue specificity :Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level)..
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type IV membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.