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Western blot analysis of extracts from Mouse Skeletal Muscle tissue lyaste.using FNDC5 Rabbit mAb [F0J4] at dilution of 1:1000 incubated at 4℃ over night
Protein names :FNDC5,FRCP2,FNDC5_HUMAN,Fibronectin type III domain-containing protein 5 [Cleaved into: Irisin]
UniProtID :Q8NAU1
MASS(da) :23,659
MW(kDa) :24 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human FNDC5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse Skeletal Muscle tissue lyaste
Function : [Irisin]: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue..
Tissue specificity :Widely expressed, with highest levels in heart. Very low expression, if any, in colon, pancreas and spleen..
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Peroxisome membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.