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NDUFS8 Rabbit mAb [7S5Y]Cat NO.: A20041

Western blot analysis of extracts from HeLa  cells lyastes.using NDUFS8 Rabbit mAb [7S5Y] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :NDUFS8,NDUS8_HUMAN,NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

UniProtID :O00217

MASS(da) :23,705

MW(kDa) :23 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NDUFS8.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa  cells lyastes

Function : Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:22499348). Essential for the catalytic activity and assembly of complex I (PubMed:22499348)..

Tissue specificity :Expressed in all tissues with the highest level in heart and skeletal muscle and the lowest level in lung..

Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.