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Western blot analysis of extracts from HeLa cells lyastes.using CAPZB Rabbit mAb [0K9X] at dilution of 1:1000 incubated at 4℃ over night
Protein names :CAPZB,CAPZB_HUMAN,F-actin-capping protein subunit beta
UniProtID :P47756
MASS(da) :31,350
MW(kDa) :31 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CAPZB.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lyastes
Function : F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization..
Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.