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Western blot analysis of extracts from Fetal heart tissue lyaste.using Perilipin-1 Rabbit mAb [J33H] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PLIN1,PERI,PLIN,PLIN1_HUMAN,Perilipin-1
UniProtID :O60240
MASS(da) :55,990
MW(kDa) :62 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Perilipin-1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Fetal heart tissue lyaste
Function : Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels..
Tissue specificity :Detected in adipocytes from white adipose tissue (at protein level) (PubMed:27832861). Detected in visceral adipose tissue and mammary gland (PubMed:9521880)..
Subcellular locationi :Endoplasmic reticulum. Lipid droplet.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.