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Western blot analysis of extracts from Human melanoma tissue lyaste.using PHLDA1 Rabbit mAb [13C8] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PHLDA1,PHRIP,TDAG51,PHLA1_HUMAN,Pleckstrin homology-like domain family A member 1
UniProtID :Q8WV24
MASS(da) :45,016
MW(kDa) :45 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PHLDA1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human melanoma tissue lyaste
Function : Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation..
Tissue specificity :Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level)..
Subcellular locationi :Cytoplasm. Cytoplasmic vesicle. Nucleus, nucleolus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.