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Collagen II Rabbit mAb [7BB9]Cat NO.: A39981

Western blot analysis of extracts from Rat cartilage tissue lyaste.using Collagen II Rabbit mAb [7BB9] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :COL2A1,CO2A1_HUMAN,Collagen alpha-1 chain [Cleaved into: Collagen alpha-1 chain,Chondrocalcin]

UniProtID :P02458

MASS(da) :141,785

MW(kDa) :141 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Collagen II.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Rat cartilage tissue lyaste

Function : Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Tissue specificity :Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte..

Subcellular locationi :Secreted, extracellular space, extracellular matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.