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NEAS Rabbit mAb [1Y0E]Cat NO.: A46040

Western blot analysis of extracts from HeLa cells lyastes.using NEAS Rabbit mAb [1Y0E] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :SPTAN1,NEAS,SPTA2,SPTN1_HUMAN,Spectrin alpha chain, non-erythrocytic 1

UniProtID :Q13813

MASS(da) :284,539

MW(kDa) :150, 285 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NEAS.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lyastes

Function : Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.