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Western blot analysis of extracts from MCF-7 cells lyastes.using Rap2A+Rap2B+Rap2C Rabbit mAb [1X3F] at dilution of 1:1000 incubated at 4℃ over night
Protein names :RAP2C,RAP2C_HUMAN,Ras-related protein Rap-2c
UniProtID :P61225,P10114,Q9Y3L5
MASS(da) :20,745
MW(kDa) :21 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Rap2A+Rap2B+Rap2C.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells lyastes
Function : Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May play a role in SRE-mediated gene transcription..
Tissue specificity :Expressed in liver, skeletal muscle, prostate, uterus, rectum, stomach, and bladder and to a lower extent in brain, kidney, pancreas, and bone marrow. Expressed in mononuclear leukocytes and megakaryocytes..
Subcellular locationi :Cytoplasm. Recycling endosome membrane,Lipid-anchor,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.