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Western blot analysis of extracts from HeLa cells lyastes.using NOP58 Rabbit mAb [H04D] at dilution of 1:1000 incubated at 4℃ over night
Protein names :NOP58,NOL5,NOP5,HSPC120,NOP58_HUMAN,Nucleolar protein 58
UniProtID :Q9Y2X3
MASS(da) :59,578
MW(kDa) :60 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NOP58.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lyastes
Function : Required for 60S ribosomal subunit biogenesis (By similarity). Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs..
Tissue specificity :Ubiquitous.
Subcellular locationi :Nucleus, nucleolus. Nucleus, nucleoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.