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Western blot analysis of extracts from HeLa cells lyastes.using POMP Rabbit mAb [U706] at dilution of 1:1000 incubated at 4℃ over night
Protein names :POMP,C13orf12,UMP1,HSPC014,HSPC036,PNAS-110,POMP_HUMAN,Proteasome maturation protein
UniProtID :Q9Y244
MASS(da) :15,789
MW(kDa) :16 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human POMP.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lyastes
Function : Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum..
Tissue specificity :Strongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer..
Subcellular locationi :Cytoplasm, cytosol. Nucleus. Microsome membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.