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Western blot analysis of extracts from Human skeletal muscle tissue lyaste.using SMYD1 Rabbit mAb [WJD5] at dilution of 1:1000 incubated at 4℃ over night
Protein names :SMYD1,SMYD1_HUMAN,Histone-lysine N-methyltransferase SMYD1
UniProtID :Q8NB12
MASS(da) :56,617
MW(kDa) :56 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human SMYD1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human skeletal muscle tissue lyaste
Function : Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis..
Tissue specificity :Expression seems mostly restricted to heart and skeletal muscle..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.