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Western blot analysis of extracts from Fetal brain tissue lyaste.using SCGN/Secretagogin Rabbit mAb [3894] at dilution of 1:1000 incubated at 4℃ over night
Protein names :SCGN,SECRET,SEGN_HUMAN,Secretagogin
UniProtID :O76038
MASS(da) :32,040
MW(kDa) :32 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human SCGN/Secretagogin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Fetal brain tissue lyaste
Tissue specificity :Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons..
Subcellular locationi :Cytoplasm. Secreted. Cytoplasmic vesicle, secretory vesicle membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.