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Western blot(SDS PAGE) analysis of extracts from NCCIT cells.Using ORF1p Rabbit mAb [N1C1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :L1RE1,LRE1,LORF1_HUMAN,LINE-1 retrotransposable element ORF1 protein
UniProtID :Q9UN81
MASS(da) :40,056
MW(kDa) :42 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human ORF1p
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NCCIT cells
Function : Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded..
Subcellular locationi :Nucleus, nucleolus. Cytoplasm, Cytoplasmic ribonucleoprotein granule. Cytoplasm, Stress granule.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.