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Western blot analysis of extracts from U87-MG cells lyastes.using Engrailed 1 Rabbit mAb [4UI7] at dilution of 1:1000 incubated at 4℃ over night
Protein names :EN1,HME1_HUMAN,Homeobox protein engrailed-1
UniProtID :Q05925
MASS(da) :40,115
MW(kDa) :55 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Engrailed 1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U87-MG cells lyastes
Function : Required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.