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Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using Lamin B2 Rabbit mAb [80S3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LMNB2,LMN2,LMNB2_HUMAN,Lamin-B2
UniProtID :Q03252
MASS(da) :69,948
MW(kDa) :68 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Lamin B2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin..
Subcellular locationi :Nucleus lamina.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.