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Western blot analysis of extracts from HeLa cells lyastes.using CPSF2/CPSF100 Rabbit mAb [94K0] at dilution of 1:1000 incubated at 4℃ over night
Protein names :CPSF2,CPSF100,KIAA1367,CPSF2_HUMAN,Cleavage and polyadenylation specificity factor subunit 2
UniProtID :Q9P2I0
MASS(da) :88,487
MW(kDa) :103 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CPSF2/CPSF100.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lyastes
Function : Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.