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Western blot analysis of extracts from A549 cells lyastes TNF alpha and TPA - treated.using CXCL5 Rabbit mAb [04U0] at dilution of 1:1000 incubated at 4℃ over night
Protein names :CXCL5,ENA78,SCYB5,CXCL5_HUMAN,C-X-C motif chemokine 5 ) [Cleaved into: ENA-78,ENA-78]
UniProtID :P42830
MASS(da) :11,972
MW(kDa) :8 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CXCL5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells lyastes TNF alpha and TPA treated
Function : Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.