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LARS Rabbit mAb [ME18]Cat NO.: A12011

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using LARS Rabbit mAb [ME18] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :LARS1,KIAA1352,LARS,SYLC_HUMAN,Leucine--tRNA ligase, cytoplasmic

UniProtID :Q9P2J5

MASS(da) :134,466

MW(kDa) :134 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human LARS

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.