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Nicalin Rabbit mAb [5NAR]Cat NO.: A32117

Western blot analysis of extracts from A431 cells lyastes.using Nicalin Rabbit mAb [5NAR] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :NCLN,NCLN_HUMAN,Nicalin

UniProtID :Q969V3

MASS(da) :62,974

MW(kDa) :60 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Nicalin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A431 cells lyastes

Function : Component of a ribosome-associated translocon complex involved in multi-pass membrane protein transport into the endoplasmic reticulum (ER) membrane and biogenesis (PubMed:32820719). May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning, via its interaction with NOMO (By similarity)..

Tissue specificity :Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart..

Subcellular locationi :Endoplasmic reticulum membrane,Single-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.