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Western blot analysis of extracts from Mouse brain tissue lyaste.using NMDAR2A Rabbit mAb [DDLP] at dilution of 1:1000 incubated at 4℃ over night
Protein names :GRIN2A,NMDAR2A,NMDE1_HUMAN,Glutamate receptor ionotropic, NMDA 2A
UniProtID :Q12879
MASS(da) :165,283
MW(kDa) :180 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NMDAR2A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain tissue lyaste
Function : Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:8768735, PubMed:26919761, PubMed:26875626, PubMed:28105280). Sensitivity to glutamate and channel kinetics depend on the subunit composition,channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B (PubMed:26919761, PubMed:26875626). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity)..
Subcellular locationi :Cell projection, dendritic spine. Cell membrane,Multi-pass membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane,Multi-pass membrane protein. Cytoplasmic vesicle membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.