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Ube1L Rabbit mAb [DG4A]Cat NO.: A86394

Western blot analysis of extracts from HL60 cells lyastes.using Ube1L Rabbit mAb [DG4A] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :UBA7,UBE1L,UBE2,UBA7_HUMAN,Ubiquitin-like modifier-activating enzyme 7

UniProtID :P41226

MASS(da) :111,694

MW(kDa) :111 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Ube1L.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HL60 cells lyastes

Function : Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein..

Tissue specificity :Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.