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p50 dynamitin Rabbit mAb [YA50]Cat NO.: A87822

Western blot analysis of extracts from MCF-7 cells lyastes.using p50 dynamitin Rabbit mAb [YA50] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :DCTN2,DCTN50,DCTN2_HUMAN,Dynactin subunit 2

UniProtID :Q13561

MASS(da) :44,231

MW(kDa) :44 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human p50 dynamitin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :MCF-7 cells lyastes

Function : Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.

Subcellular locationi :Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Membrane,Peripheral membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.