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Western blot analysis of extracts from Recombinant protein .using Osteocalcin Rabbit mAb [W1F5] at dilution of 1:1000 incubated at 4℃ over night
Protein names :BGLAP,OSTCN_HUMAN,Osteocalcin
UniProtID :P02818
MASS(da) :10,963
MW(kDa) :11 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Osteocalcin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Recombinant protein
Function : Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.