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HACE1 Rabbit mAb [5I26]Cat NO.: A81491

Western blot analysis of extracts from 293T cells lyastes.using HACE1 Rabbit mAb [5I26] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :HACE1,KIAA1320,HACE1_HUMAN,E3 ubiquitin-protein ligase HACE1

UniProtID :Q8IYU2

MASS(da) :102,342

MW(kDa) :102 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human HACE1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cells lyastes

Function : E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB..

Tissue specificity :Expressed in multiple tissues including heart, brain and kidney..

Subcellular locationi :Golgi apparatus, Golgi stack membrane. Cytoplasm. Endoplasmic reticulum.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.