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Western blot analysis of extracts from 293T cells lyastes.using HACE1 Rabbit mAb [5I26] at dilution of 1:1000 incubated at 4℃ over night
Protein names :HACE1,KIAA1320,HACE1_HUMAN,E3 ubiquitin-protein ligase HACE1
UniProtID :Q8IYU2
MASS(da) :102,342
MW(kDa) :102 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human HACE1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells lyastes
Function : E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB..
Tissue specificity :Expressed in multiple tissues including heart, brain and kidney..
Subcellular locationi :Golgi apparatus, Golgi stack membrane. Cytoplasm. Endoplasmic reticulum.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.