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LC3A/B Rabbit mAb [L85C]Cat NO.: A59552

Western blot(SDS PAGE) analysis of extracts from RD cells Torin 1-treated (250 nM 4 hr;).Using LC3A/B Rabbit mAb [L85C] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MAP1LC3B,MAP1ALC3,MLP3B_HUMAN,Microtubule-associated proteins 1A/1B light chain 3B

UniProtID :Q9GZQ8

MASS(da) :14,688

MW(kDa) :14, 16 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human LC3A/B

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :RD cells Torin 1-treated (250 nM 4 hr;)

Function : Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY

Tissue specificity :Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver..

Subcellular locationi :Cytoplasmic vesicle, autophagosome membrane,Lipid-anchor. Endomembrane system,Lipid-anchor. Mitochondrion membrane,Lipid-anchor. Cytoplasm, cytoskeleton. Cytoplasmic vesicle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.