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Western blot analysis of extracts from Jurkat cells lyastes.using NOP10 Rabbit mAb [G1ML] at dilution of 1:1000 incubated at 4℃ over night
Protein names :NOP10,NOLA3,NOP10_HUMAN,H/ACA ribonucleoprotein complex subunit 3
UniProtID :Q9NPE3
MASS(da) :7,706
MW(kDa) :7 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NOP10.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells lyastes
Function : Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (
Subcellular locationi :Nucleus, nucleolus. Nucleus, Cajal body.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.