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Western blot analysis of extracts from LNCaP cells lyastes.using RNF14 Rabbit mAb [5H5L] at dilution of 1:1000 incubated at 4℃ over night
Protein names :RNF14,ARA54,HRIHFB2038,RNF14_HUMAN,E3 ubiquitin-protein ligase RNF14
UniProtID :Q9UBS8
MASS(da) :53,837
MW(kDa) :54 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human RNF14.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells lyastes
Function : Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription..
Tissue specificity :Widely expressed.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.