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RNF128/GRAIL Rabbit mAb [V5U3]Cat NO.: A29010

Western blot analysis of extracts from HeLa cells lyastes.using RNF128/GRAIL Rabbit mAb [V5U3] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :RNF128,RN128_HUMAN,E3 ubiquitin-protein ligase RNF128

UniProtID :Q8TEB7

MASS(da) :46,521

MW(kDa) :47 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human RNF128/GRAIL.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lyastes

Function : E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm,sensitizes ectoderm to respond to neural-inducing signals..

Subcellular locationi :Endomembrane system,Single-pass membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.