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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using LDHA Rabbit mAb [I016] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LDHA,PIG19,LDHA_HUMAN,L-lactate dehydrogenase A chain
UniProtID :P00338
MASS(da) :36,689
MW(kDa) :37 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human LDHA
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)..
Tissue specificity :Predominantly expressed in anaerobic tissues such as skeletal muscle and liver..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.