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Western blot analysis of extracts from Human plasma tissue lyaste.using Apolipoprotein B Rabbit mAb [745P] at dilution of 1:1000 incubated at 4℃ over night
Protein names :APOB,APOB_HUMAN,Apolipoprotein B-100 [Cleaved into: Apolipoprotein B-48 ]
UniProtID :P04114
MASS(da) :515,605
MW(kDa) :515 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Apolipoprotein B.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human plasma tissue lyaste
Function : Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Subcellular locationi :Cytoplasm. Secreted. Lipid droplet.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.