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TFIIE alpha/GTF2E1 Rabbit mAb [J3MJ]Cat NO.: A88838

Western blot analysis of extracts from Molt-4 cells lyastes.using TFIIE alpha/GTF2E1 Rabbit mAb [J3MJ] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :GTF2E1,TF2E1,T2EA_HUMAN,General transcription factor IIE subunit 1

UniProtID :P29083

MASS(da) :49,452

MW(kDa) :49 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human TFIIE alpha/GTF2E1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Molt-4 cells lyastes

Function : Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.