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IARS1 Rabbit mAb [38JY]Cat NO.: A71563

Western blot analysis of extracts from HeLa cells lyastes.using IARS1 Rabbit mAb [38JY] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :IARS1,IARS,SYIC_HUMAN,Isoleucine--tRNA ligase, cytoplasmic

UniProtID :P41252

MASS(da) :144,498

MW(kDa) :145 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human IARS1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lyastes

Function : Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA..

Tissue specificity :Expressed in liver and muscle (at protein level)..

Subcellular locationi :Cytoplasm. Cytoplasm, cytosol.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.