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Western blot analysis of extracts from Human placenta tissue lyaste.using GBGT1 Rabbit mAb [RF5X] at dilution of 1:1000 incubated at 4℃ over night
Protein names :GBGT1,UNQ2513/PRO6002,GBGT1_HUMAN,Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
UniProtID :Q8N5D6
MASS(da) :40,127
MW(kDa) :40 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human GBGT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human placenta tissue lyaste
Function : Has lost the ability to synthesize Forssman glycolipid antigen (FORS1/FG) (PubMed:10506200). Might have acquired an alternative function in glycosphingolipid metabolism, but it remains to be established. It appears to have drifted more slowly than confirmed pseudogenes in the glycosyltransferase 6 family, suggesting that it has remained under evolutionary pressure..
Tissue specificity :Widely expressed. Expressed at higher level in placenta, ovary and peripheral blood leukocyte, whereas it is weakly expressed in liver, thymus, and testis (PubMed:10506200). Expressed in bone marrow erythroid cells (PubMed:23255552)..
Subcellular locationi :Golgi apparatus membrane,Single-pass type II membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.