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Western blot analysis of extracts from Fetal brain tissue lyaste.using ACOT7 Rabbit mAb [X961] at dilution of 1:1000 incubated at 4℃ over night
Protein names :ACOT7,BACH,BACH_HUMAN,Cytosolic acyl coenzyme A thioester hydrolase
UniProtID :O00154
MASS(da) :41,796
MW(kDa) :42 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ACOT7.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Fetal brain tissue lyaste
Function : Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:10578051). Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051)..
Tissue specificity :Isoform 4 is expressed exclusively in brain..
Subcellular locationi :[Isoform 4]: Cytoplasm, cytosol.,[Isoform 6]: Cytoplasm, cytosol.,[Isoform 1]: Mitochondrion.,[Isoform 5]: Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.