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Western blot analysis of extracts from Caco-2 cells lyastes.using UGT1A6 Rabbit mAb [95K2] at dilution of 1:1000 incubated at 4℃ over night
Protein names :UGT1A6,GNT1,UGT1,UD16_HUMAN,UDP-glucuronosyltransferase 1-6
UniProtID :P19224
MASS(da) :60,751
MW(kDa) :61 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human UGT1A6.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Caco-2 cells lyastes
Function : UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. Isoform 3 lacks transferase activity but acts as a negative regulator of isoform 1 (By similarity)..
Tissue specificity :Expressed in skin. Isoforms 1 and 3 are expressed in kidney and liver. Isoform 1 but not isoform 2 is expressed in colon, esophagus and small intestine..
Subcellular locationi :Microsome. Endoplasmic reticulum membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.