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Western blot analysis of extracts from BxPC-3 cells lyastes.using VMAT1 Rabbit mAb [T2Q7] at dilution of 1:1000 incubated at 4℃ over night
Protein names :SLC18A1,VAT1,VMAT1,VMAT1_HUMAN,Chromaffin granule amine transporter
UniProtID :P54219
MASS(da) :56,257
MW(kDa) :56 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human VMAT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :BxPC-3 cells lyastes
Function : Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells..
Tissue specificity :Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level)..
Subcellular locationi :[Isoform 1]: Cytoplasmic vesicle membrane,Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane,Multi-pass membrane protein.,[Isoform 2]: Endoplasmic reticulum membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.