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ME3 Rabbit mAb [4V0T]Cat NO.: A14514

Western blot analysis of extracts from Fetal brain tissue lyaste.using ME3 Rabbit mAb [4V0T] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :ME3,MAON_HUMAN,NADP-dependent malic enzyme, mitochondrial

UniProtID :Q16798

MASS(da) :67,068

MW(kDa) :67 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ME3.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Fetal brain tissue lyaste

Function : Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor (PubMed:7818469). Can also reverse the decarboxylation reaction, but only with significantly lower efficiency (PubMed:7818469)..

Tissue specificity :Expressed predominantly in organs with a low-division rate.

Subcellular locationi :Mitochondrion matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.