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EF-Ts Rabbit mAb [U7I1]Cat NO.: A39975

Western blot analysis of extracts from HeLa cells lyastes.using EF-Ts Rabbit mAb [U7I1] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :TSFM,EFTS_HUMAN,Elongation factor Ts, mitochondrial

UniProtID :P43897

MASS(da) :35,391

MW(kDa) :35 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human EF-Ts.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lyastes

Function : Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome..

Tissue specificity :Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney.

Subcellular locationi :Mitochondrion.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.