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Western blot analysis of extracts from MOLT4 cells lyastes.using ZWINT Rabbit mAb [4OFP] at dilution of 1:1000 incubated at 4℃ over night
Protein names :ZWINT,ZWINT_HUMAN,ZW10 interactor
UniProtID :O95229
MASS(da) :31,293
MW(kDa) :31 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ZWINT.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MOLT4 cells lyastes
Function : Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase..
Subcellular locationi :Nucleus. Chromosome, centromere, kinetochore.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.