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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using LSR Rabbit mAb [IBM1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LSR,ILDR3,LISCH,LSR_HUMAN,Lipolysis-stimulated lipoprotein receptor
UniProtID :Q86X29
MASS(da) :71,439
MW(kDa) :65 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human LSR
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells (By similarity). Maintains epithelial barrier function by recruiting MARVELD2/tricellulin to tricellular tight junctions (By similarity)..
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Cell junction, tight junction.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.