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Western blot analysis of extracts from A431 cells lyastes.using PHKA1 Rabbit mAb [4JV3] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PHKA1,PHKA,KPB1_HUMAN,Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
UniProtID :P46020
MASS(da) :137,312
MW(kDa) :137 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PHKA1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells lyastes
Function : Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
Tissue specificity :Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.
Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.