support:13376277882 | tech@naturebios.com

PHKA1 Rabbit mAb [4JV3]Cat NO.: A92621

Western blot analysis of extracts from A431 cells lyastes.using PHKA1 Rabbit mAb [4JV3] at dilution of 1:1000 incubated at 4℃ over night

  • SizePriceIn stock
  • 50ul1280咨询客服
  • 100ul2380咨询客服
Order Datasheet

Product information

Protein names :PHKA1,PHKA,KPB1_HUMAN,Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

UniProtID :P46020

MASS(da) :137,312

MW(kDa) :137 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PHKA1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A431 cells lyastes

Function : Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

Tissue specificity :Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.

Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.