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Western blot analysis of extracts from HT29 cells lyastes.using CTU2/NCS2 Rabbit mAb [668H] at dilution of 1:1000 incubated at 4℃ over night
Protein names :CTU2,C16orf84,NCS2,CTU2_HUMAN,Cytoplasmic tRNA 2-thiolation protein 2
UniProtID :Q2VPK5
MASS(da) :56,107
MW(kDa) :56 kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CTU2/NCS2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT29 cells lyastes
Function : Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.