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CTU2/NCS2 Rabbit mAb [668H]Cat NO.: A27018

Western blot analysis of extracts from HT29 cells lyastes.using CTU2/NCS2 Rabbit mAb [668H] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :CTU2,C16orf84,NCS2,CTU2_HUMAN,Cytoplasmic tRNA 2-thiolation protein 2

UniProtID :Q2VPK5

MASS(da) :56,107

MW(kDa) :56 kda

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CTU2/NCS2.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HT29 cells lyastes

Function : Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.