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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using MACC1 Rabbit mAb [5161] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MACC1,MACC1_HUMAN,Metastasis-associated in colon cancer protein 1
UniProtID :Q6ZN28
MASS(da) :96,639
MW(kDa) :97 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human MACC1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo..
Tissue specificity :Preferentially expressed in metastasizing tumors..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.